Enhanced arsenic tolerance of transgenic eastern cottonwood plants expressing gamma-glutamylcysteine synthetase.
Identifieur interne : 002F03 ( Main/Exploration ); précédent : 002F02; suivant : 002F04Enhanced arsenic tolerance of transgenic eastern cottonwood plants expressing gamma-glutamylcysteine synthetase.
Auteurs : Melissa S. Leblanc [États-Unis] ; Amparo Lima ; Paul Montello ; Tehryoung Kim ; Richard B. Meagher ; Scott MerkleSource :
- International journal of phytoremediation [ 1522-6514 ] ; 2011.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Arabidopsis (génétique), Arsenic (analyse), Arsenic (métabolisme), Arsenic (toxicité), Dépollution biologique de l'environnement (MeSH), Escherichia coli (enzymologie), Escherichia coli (génétique), Glutamate-cysteine ligase (génétique), Glutamate-cysteine ligase (métabolisme), Populus (croissance et développement), Populus (effets des médicaments et des substances chimiques), Populus (enzymologie), Populus (génétique), Pousses de plante (composition chimique), Pousses de plante (croissance et développement), Pousses de plante (effets des médicaments et des substances chimiques), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Racines de plante (composition chimique), Racines de plante (croissance et développement), Racines de plante (effets des médicaments et des substances chimiques), Régions promotrices (génétique) (génétique), Régulation de l'expression des gènes codant pour des enzymes (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Techniques de culture de tissus (MeSH), Thiols (métabolisme), Végétaux génétiquement modifiés (MeSH).
- MESH :
- analyse : Arsenic.
- composition chimique : Pousses de plante, Racines de plante.
- croissance et développement : Populus, Pousses de plante, Racines de plante.
- effets des médicaments et des substances chimiques : Populus, Pousses de plante, Racines de plante.
- enzymologie : Escherichia coli, Populus.
- génétique : Agrobacterium tumefaciens, Arabidopsis, Escherichia coli, Glutamate-cysteine ligase, Populus, Protéines bactériennes, Régions promotrices (génétique).
- métabolisme : Arsenic, Glutamate-cysteine ligase, Protéines bactériennes, Thiols.
- toxicité : Arsenic.
- Dépollution biologique de l'environnement, Régulation de l'expression des gènes codant pour des enzymes, Régulation de l'expression des gènes végétaux, Techniques de culture de tissus, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Arabidopsis (genetics), Arsenic (analysis), Arsenic (metabolism), Arsenic (toxicity), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Biodegradation, Environmental (MeSH), Escherichia coli (enzymology), Escherichia coli (genetics), Gene Expression Regulation, Enzymologic (MeSH), Gene Expression Regulation, Plant (MeSH), Glutamate-Cysteine Ligase (genetics), Glutamate-Cysteine Ligase (metabolism), Plant Roots (chemistry), Plant Roots (drug effects), Plant Roots (growth & development), Plant Shoots (chemistry), Plant Shoots (drug effects), Plant Shoots (growth & development), Plants, Genetically Modified (MeSH), Populus (drug effects), Populus (enzymology), Populus (genetics), Populus (growth & development), Promoter Regions, Genetic (genetics), Sulfhydryl Compounds (metabolism), Tissue Culture Techniques (MeSH).
- MESH :
- chemical , analysis : Arsenic.
- chemistry : Plant Roots, Plant Shoots.
- drug effects : Plant Roots, Plant Shoots, Populus.
- enzymology : Escherichia coli, Populus.
- genetics : Agrobacterium tumefaciens, Arabidopsis, Bacterial Proteins, Escherichia coli, Glutamate-Cysteine Ligase, Populus, Promoter Regions, Genetic.
- growth & development : Plant Roots, Plant Shoots, Populus.
- chemical , metabolism : Arsenic, Bacterial Proteins, Glutamate-Cysteine Ligase, Sulfhydryl Compounds.
- chemical , toxicity : Arsenic.
- Biodegradation, Environmental, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Plants, Genetically Modified, Tissue Culture Techniques.
Abstract
Arsenic is a metalloid that occurs naturally at parts per million (ppm) levels in the earth's crust. Natural and human activities have contributed to arsenic mobilization and increased concentration in the environment, such that World Health Organization guidelines for arsenic levels in drinking water are exceeded at many locations, worldwide. This translates into an increased risk of arsenic-related illnesses for millions of people. Recent studies demonstrate that increasing thiol-sinks in transgenic plants by overexpressing the bacterial gamma-glutamylcysteine synthetase (ECS) gene results in a higher tolerance and accumulation of metals and metalloids such as cadmium, mercury, and arsenic. We used Agrobacterium-mediated transformation to genetically engineer eastern cottonwood with a bacterial ECS gene. Eastern cottonwood plants expressing ECS had elevated thiol group levels, consistent with increased ECS activity. In addition, these ECS-expressing plants had enhanced growth on levels of arsenate toxic to control plants in vitro. Furthermore, roots of ECS-expressing plants accumulated significantly more arsenic than control roots (approximately twice as much), while shoots accumulated significantly less arsenic than control shoots (approximately two-thirds as much). We discuss potential mechanisms for shifting the balance of plant arsenic distribution from root accumulation to shoot accumulation, as it pertains to arsenic phytoremediation.
DOI: 10.1080/15226514.2010.499917
PubMed: 21972493
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Enhanced arsenic tolerance of transgenic eastern cottonwood plants expressing gamma-glutamylcysteine synthetase.</title><author><name sortKey="Leblanc, Melissa S" sort="Leblanc, Melissa S" uniqKey="Leblanc M" first="Melissa S" last="Leblanc">Melissa S. Leblanc</name><affiliation wicri:level="2"><nlm:affiliation>Genetics Department, University of Georgia, Athens, GA 30602, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Genetics Department, University of Georgia, Athens, GA 30602</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Lima, Amparo" sort="Lima, Amparo" uniqKey="Lima A" first="Amparo" last="Lima">Amparo Lima</name></author><author><name sortKey="Montello, Paul" sort="Montello, Paul" uniqKey="Montello P" first="Paul" last="Montello">Paul Montello</name></author><author><name sortKey="Kim, Tehryoung" sort="Kim, Tehryoung" uniqKey="Kim T" first="Tehryoung" last="Kim">Tehryoung Kim</name></author><author><name sortKey="Meagher, Richard B" sort="Meagher, Richard B" uniqKey="Meagher R" first="Richard B" last="Meagher">Richard B. Meagher</name></author><author><name sortKey="Merkle, Scott" sort="Merkle, Scott" uniqKey="Merkle S" first="Scott" last="Merkle">Scott Merkle</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21972493</idno><idno type="pmid">21972493</idno><idno type="doi">10.1080/15226514.2010.499917</idno><idno type="wicri:Area/Main/Corpus">002C72</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002C72</idno><idno type="wicri:Area/Main/Curation">002C72</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002C72</idno><idno type="wicri:Area/Main/Exploration">002C72</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Enhanced arsenic tolerance of transgenic eastern cottonwood plants expressing gamma-glutamylcysteine synthetase.</title><author><name sortKey="Leblanc, Melissa S" sort="Leblanc, Melissa S" uniqKey="Leblanc M" first="Melissa S" last="Leblanc">Melissa S. Leblanc</name><affiliation wicri:level="2"><nlm:affiliation>Genetics Department, University of Georgia, Athens, GA 30602, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Genetics Department, University of Georgia, Athens, GA 30602</wicri:regionArea><placeName><region type="state">Géorgie (États-Unis)</region></placeName></affiliation></author><author><name sortKey="Lima, Amparo" sort="Lima, Amparo" uniqKey="Lima A" first="Amparo" last="Lima">Amparo Lima</name></author><author><name sortKey="Montello, Paul" sort="Montello, Paul" uniqKey="Montello P" first="Paul" last="Montello">Paul Montello</name></author><author><name sortKey="Kim, Tehryoung" sort="Kim, Tehryoung" uniqKey="Kim T" first="Tehryoung" last="Kim">Tehryoung Kim</name></author><author><name sortKey="Meagher, Richard B" sort="Meagher, Richard B" uniqKey="Meagher R" first="Richard B" last="Meagher">Richard B. Meagher</name></author><author><name sortKey="Merkle, Scott" sort="Merkle, Scott" uniqKey="Merkle S" first="Scott" last="Merkle">Scott Merkle</name></author></analytic><series><title level="j">International journal of phytoremediation</title><idno type="ISSN">1522-6514</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Arabidopsis (genetics)</term><term>Arsenic (analysis)</term><term>Arsenic (metabolism)</term><term>Arsenic (toxicity)</term><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (metabolism)</term><term>Biodegradation, Environmental (MeSH)</term><term>Escherichia coli (enzymology)</term><term>Escherichia coli (genetics)</term><term>Gene Expression Regulation, Enzymologic (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Glutamate-Cysteine Ligase (genetics)</term><term>Glutamate-Cysteine Ligase (metabolism)</term><term>Plant Roots (chemistry)</term><term>Plant Roots (drug effects)</term><term>Plant Roots (growth & development)</term><term>Plant Shoots (chemistry)</term><term>Plant Shoots (drug effects)</term><term>Plant Shoots (growth & development)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (drug effects)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Promoter Regions, Genetic (genetics)</term><term>Sulfhydryl Compounds (metabolism)</term><term>Tissue Culture Techniques (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (génétique)</term><term>Arabidopsis (génétique)</term><term>Arsenic (analyse)</term><term>Arsenic (métabolisme)</term><term>Arsenic (toxicité)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Escherichia coli (enzymologie)</term><term>Escherichia coli (génétique)</term><term>Glutamate-cysteine ligase (génétique)</term><term>Glutamate-cysteine ligase (métabolisme)</term><term>Populus (croissance et développement)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Pousses de plante (composition chimique)</term><term>Pousses de plante (croissance et développement)</term><term>Pousses de plante (effets des médicaments et des substances chimiques)</term><term>Protéines bactériennes (génétique)</term><term>Protéines bactériennes (métabolisme)</term><term>Racines de plante (composition chimique)</term><term>Racines de plante (croissance et développement)</term><term>Racines de plante (effets des médicaments et des substances chimiques)</term><term>Régions promotrices (génétique) (génétique)</term><term>Régulation de l'expression des gènes codant pour des enzymes (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Techniques de culture de tissus (MeSH)</term><term>Thiols (métabolisme)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Arsenic</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Arsenic</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Roots</term><term>Plant Shoots</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Pousses de plante</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term><term>Pousses de plante</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Plant Roots</term><term>Plant Shoots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Populus</term><term>Pousses de plante</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Escherichia coli</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Escherichia coli</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Arabidopsis</term><term>Bacterial Proteins</term><term>Escherichia coli</term><term>Glutamate-Cysteine Ligase</term><term>Populus</term><term>Promoter Regions, Genetic</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Roots</term><term>Plant Shoots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Arabidopsis</term><term>Escherichia coli</term><term>Glutamate-cysteine ligase</term><term>Populus</term><term>Protéines bactériennes</term><term>Régions promotrices (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Arsenic</term><term>Bacterial Proteins</term><term>Glutamate-Cysteine Ligase</term><term>Sulfhydryl Compounds</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arsenic</term><term>Glutamate-cysteine ligase</term><term>Protéines bactériennes</term><term>Thiols</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Arsenic</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Arsenic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Gene Expression Regulation, Enzymologic</term><term>Gene Expression Regulation, Plant</term><term>Plants, Genetically Modified</term><term>Tissue Culture Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Dépollution biologique de l'environnement</term><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Régulation de l'expression des gènes végétaux</term><term>Techniques de culture de tissus</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Arsenic is a metalloid that occurs naturally at parts per million (ppm) levels in the earth's crust. Natural and human activities have contributed to arsenic mobilization and increased concentration in the environment, such that World Health Organization guidelines for arsenic levels in drinking water are exceeded at many locations, worldwide. This translates into an increased risk of arsenic-related illnesses for millions of people. Recent studies demonstrate that increasing thiol-sinks in transgenic plants by overexpressing the bacterial gamma-glutamylcysteine synthetase (ECS) gene results in a higher tolerance and accumulation of metals and metalloids such as cadmium, mercury, and arsenic. We used Agrobacterium-mediated transformation to genetically engineer eastern cottonwood with a bacterial ECS gene. Eastern cottonwood plants expressing ECS had elevated thiol group levels, consistent with increased ECS activity. In addition, these ECS-expressing plants had enhanced growth on levels of arsenate toxic to control plants in vitro. Furthermore, roots of ECS-expressing plants accumulated significantly more arsenic than control roots (approximately twice as much), while shoots accumulated significantly less arsenic than control shoots (approximately two-thirds as much). We discuss potential mechanisms for shifting the balance of plant arsenic distribution from root accumulation to shoot accumulation, as it pertains to arsenic phytoremediation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21972493</PMID><DateCompleted><Year>2012</Year><Month>08</Month><Day>09</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1522-6514</ISSN><JournalIssue CitedMedium="Print"><Volume>13</Volume><Issue>7</Issue><PubDate><Year>2011</Year><Month>Aug</Month></PubDate></JournalIssue><Title>International journal of phytoremediation</Title><ISOAbbreviation>Int J Phytoremediation</ISOAbbreviation></Journal><ArticleTitle>Enhanced arsenic tolerance of transgenic eastern cottonwood plants expressing gamma-glutamylcysteine synthetase.</ArticleTitle><Pagination><MedlinePgn>657-73</MedlinePgn></Pagination><Abstract><AbstractText>Arsenic is a metalloid that occurs naturally at parts per million (ppm) levels in the earth's crust. Natural and human activities have contributed to arsenic mobilization and increased concentration in the environment, such that World Health Organization guidelines for arsenic levels in drinking water are exceeded at many locations, worldwide. This translates into an increased risk of arsenic-related illnesses for millions of people. Recent studies demonstrate that increasing thiol-sinks in transgenic plants by overexpressing the bacterial gamma-glutamylcysteine synthetase (ECS) gene results in a higher tolerance and accumulation of metals and metalloids such as cadmium, mercury, and arsenic. We used Agrobacterium-mediated transformation to genetically engineer eastern cottonwood with a bacterial ECS gene. Eastern cottonwood plants expressing ECS had elevated thiol group levels, consistent with increased ECS activity. In addition, these ECS-expressing plants had enhanced growth on levels of arsenate toxic to control plants in vitro. Furthermore, roots of ECS-expressing plants accumulated significantly more arsenic than control roots (approximately twice as much), while shoots accumulated significantly less arsenic than control shoots (approximately two-thirds as much). We discuss potential mechanisms for shifting the balance of plant arsenic distribution from root accumulation to shoot accumulation, as it pertains to arsenic phytoremediation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>LeBlanc</LastName><ForeName>Melissa S</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Genetics Department, University of Georgia, Athens, GA 30602, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lima</LastName><ForeName>Amparo</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Montello</LastName><ForeName>Paul</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Tehryoung</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Meagher</LastName><ForeName>Richard B</ForeName><Initials>RB</Initials></Author><Author ValidYN="Y"><LastName>Merkle</LastName><ForeName>Scott</ForeName><Initials>S</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>1F32ES015414-01</GrantID><Acronym>ES</Acronym><Agency>NIEHS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Int J Phytoremediation</MedlineTA><NlmUniqueID>101136878</NlmUniqueID><ISSNLinking>1522-6514</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013438">Sulfhydryl Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 6.3.2.2</RegistryNumber><NameOfSubstance UI="D005721">Glutamate-Cysteine Ligase</NameOfSubstance></Chemical><Chemical><RegistryNumber>N712M78A8G</RegistryNumber><NameOfSubstance UI="D001151">Arsenic</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001151" MajorTopicYN="N">Arsenic</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015971" MajorTopicYN="N">Gene Expression Regulation, Enzymologic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005721" MajorTopicYN="N">Glutamate-Cysteine Ligase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018520" MajorTopicYN="N">Plant Shoots</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013438" MajorTopicYN="N">Sulfhydryl Compounds</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046509" MajorTopicYN="N">Tissue Culture Techniques</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>10</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>10</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>8</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21972493</ArticleId><ArticleId IdType="doi">10.1080/15226514.2010.499917</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><noCountry><name sortKey="Kim, Tehryoung" sort="Kim, Tehryoung" uniqKey="Kim T" first="Tehryoung" last="Kim">Tehryoung Kim</name><name sortKey="Lima, Amparo" sort="Lima, Amparo" uniqKey="Lima A" first="Amparo" last="Lima">Amparo Lima</name><name sortKey="Meagher, Richard B" sort="Meagher, Richard B" uniqKey="Meagher R" first="Richard B" last="Meagher">Richard B. Meagher</name><name sortKey="Merkle, Scott" sort="Merkle, Scott" uniqKey="Merkle S" first="Scott" last="Merkle">Scott Merkle</name><name sortKey="Montello, Paul" sort="Montello, Paul" uniqKey="Montello P" first="Paul" last="Montello">Paul Montello</name></noCountry><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Leblanc, Melissa S" sort="Leblanc, Melissa S" uniqKey="Leblanc M" first="Melissa S" last="Leblanc">Melissa S. Leblanc</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002F03 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002F03 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:21972493
|texte= Enhanced arsenic tolerance of transgenic eastern cottonwood plants expressing gamma-glutamylcysteine synthetase.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21972493" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |